Fig 3.

Effect of EDTA and phosphate on V. fischeri DNase activity. (A) Wild-type (ES114) cells were grown with shaking in LBS to an OD₆₀₀ of 0.5 and 1.0. Cell-free supernatants with and without added EDTA (10 mmol/L) were mixed with DNaseAlert reagents to measure nuclease activity. Each bar represents the average of five replicates (with standard error); these results are representative of three separate experiments. (B) Cells were grown in a minimal medium containing either 0.3 or 3 mM phosphate and then assayed for nuclease activity as described previously. Each bar represents the average of five replicates from three experiments (with standard error). Different letters indicate significant differences based on ANOVA. (P-value < 0.05).