Fig 4.

Degradation of eDNA by V. fischeri strains. Strains were cultured in Tris-minimal medium (TMM) to an OD₆₀₀ of approximately 2.0. Either sterile TMM or cell-free supernatant from each strain was mixed with (A) ~0.2 µg of PCR DNA (linear) or (B) 1 µg of plasmid (pUC19) incubated at 28°C for the times (in minutes) indicated, and then visualized on a 1% agarose gel.