Single-strain squid colonization. (A) Closed squares, closed triangles, and open circles represent bioluminescence emission per squid infected with either xds, dns, or the wild-type strain ES114, respectively. Red lines represent average relative light units (RLU) per squid over the 48 hours. The number of colonized squid was determined by comparing RLU emissions between aposymbiotic squid and the test strains. (B) Nuclease is not required for normal growth or luminescence in culture. All results shown are typical for three independent trials.