Fig 2.

Improvement of the GA titer based on miox mutation. (A) Analysis of the difference of GA production caused by miox mutation based on TFB-GA output. The control represents E. coli BL21 (DE3) with GA production plasmid pRSF-MIU. The M6 and M33 represent E. coli BL21 (DE3) with GA production mutant plasmid pRSF-M-MIU. The TFB-GA output of M6 and M33 was higher than that of the control strain, and they were selected for further fermentation to determine the GA titer. (B) The optical density at 600 nm of control, M6, and M33 strains. (C) The GA titer of control, M6, and M33 strains. Data represent the mean and standard deviation for two replicates.