TABLE 2.
Yeast strainsa
| Strain | Genotype | Description/phenotype | Origin/reference |
|---|---|---|---|
| Wild-type strains | |||
| S288c | Wild-type S. cerevisiae strain |
Widely used laboratory strain. | (48) |
| Sb.P | Wild-type S. boulardii strain |
Natural isolate accumulating unusually high levels of acetic acid in YPD2%, sensitive to low pH. | Lene Jespersen, University of Copenhagen, Denmark |
| Enterol (ENT) | Wild-type S. boulardii strain |
Commercial S. boulardii. Shows transient acetic acid production in YPD2%. | Isolated from Pharmacy product Enterol (23) |
| UL | Wild-type S. boulardii strain |
(23, 31) | Niederberger, Nestlé (31) |
| 7103 | Wild-type S. boulardii strain |
(23, 40) | Ultra-Levure batch 7103, Laboratoires Biocodex |
| Genetically modified strains | |||
| Sb.P SDH1Sc | SDH1Y202H, Y31F/ SDH1Y202H, Y31F | Sb.P harboring two copies of the SDH1 allele of S288c. Does not accumulate acetic acid. | (23) |
| SbP ADH2-OE | TEFp-ADH2-CYC1t::IS2.1 | SbP strain harboring the ADH2 overexpression construct at the integration site IS2.1. | This work |
| SbP ADH3-OE | TEFp-ADH3-CYC1t::IS2.1 | SbP strain harboring the ADH3 overexpression construct at the integration site IS2.1. | This work |
| SbP ALD4-OE | TEFp-ALD4-CYC1t::IS2.1 | SbP strain harboring the ALD4 overexpression construct at the integration site IS2.1. | This work |
| SbP ALD5-OE | TEFp-ALD5-CYC1t::IS2.1 | SbP strain harboring the ALD5 overexpression construct at the integration site IS2.1. | This work |
| SbP ALD6-OE | TEFp-ALD6-CYC1t::IS2.1 | SbP strain harboring the ALD6 overexpression construct at the integration site IS2.1. | This work |
| Sb.P tor1ΔΔ | tor1ΔΔ | SbP strain in which the TOR1 ORF has been deleted. | This work |
| Sb.P cit1ΔΔ | cit1ΔΔ | SbP strain in which the CIT1 ORF has been deleted. | This work |
| Sb.P cit2ΔΔ | cit2ΔΔ | SbP strain in which the CIT2 ORF has been deleted. | This work |
| Sb.P cit3ΔΔ | cit3ΔΔ | SbP strain in which the CIT3 ORF has been deleted. | This work |
| Sb.P acs1ΔΔ | acs1ΔΔ | SbP strain in which the ACS1 ORF has been deleted. | This work |
| Sb.P ach1ΔΔ | ach1ΔΔ | SbP strain in which the ACH1 ORF has been deleted. | This work |
| Sb.P sdh1ΔΔ | sdh1ΔΔ | SbP strain in which the SDH1 ORF has been deleted. | This work |
| Sb.P sdh1bΔΔ | sdh1bΔΔ | SbP strain in which the SDH1b ORF has been deleted. | This work |
| ENT ADH2-OE | TEFp-ADH2-CYC1t::IS2.1 | ENT strain harboring the ADH2 overexpression construct at the integration site IS2.1. | This work |
| ENT ADH3-OE | TEFp-ADH3-CYC1t::IS2.1 | ENT strain harboring the ADH3 overexpression construct at the integration site IS2.1. | This work |
| ENT ALD4-OE | TEFp-ALD4-CYC1t::IS2.1 | ENT strain harboring the ALD4 overexpression construct at the integration site IS2.1. | This work |
| ENT ALD5-OE | TEFp-ALD5-CYC1t::IS2.1 | ENT strain harboring the ALD5 overexpression construct at the integration site IS2.1. | This work |
| ENT ALD6-OE | TEFp-ALD6-CYC1t::IS2.1 | ENT strain harboring the ALD6 overexpression construct at the integration site IS2.1. | This work |
| ENT tor1ΔΔ | tor1ΔΔ | ENT strain in which the TOR1 ORF has been deleted. | This work |
| ENT cit1ΔΔ | cit1ΔΔ | ENT strain in which the CIT1 ORF has been deleted. | This work |
| ENT cit2ΔΔ | cit2ΔΔ | ENT strain in which the CIT2 ORF has been deleted. | This work |
| ENT cit3ΔΔ | cit3ΔΔ | ENT strain in which the CIT3 ORF has been deleted. | This work |
| ENT acs1ΔΔ | acs1ΔΔ | ENT strain in which the ACS1 ORF has been deleted. | This work |
| ENT ach1ΔΔ Alias ENT1 |
ach1ΔΔ | ENT strain in which the ACH1 ORF has been deleted. | This work |
| ENT sdh1ΔΔ | sdh1ΔΔ | ENT strain in which the SDH1 ORF has been deleted. | This work |
| ENT1 | ach1ΔΔ | High acetic acid in YPD2%, does not accumulate acetic acid in YPD0.8%. | This work |
| ENT2 |
ach1ΔΔ TEFp-ALD4- CYC1t::IS2.1 |
High acetic acid in YPD2%, transient accumulation in YPD0.8%. | This work |
| ENT3 | ACH1ΔΔ TEFp-ALD4- CYC1t::IS2.1 TEFp-ALD4- CYC1t::IS7.1 |
Very high acetic acid in YPD2%, permanent acetic acid accumulation in YPD0.8% | This work |
a
The cassette TEFp-ALD4- CYC1t, present in the strains ENT2; ENT3 is referred to as ALD4-OE.