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. 2024 Jun 17;132(6):067005. doi: 10.1289/EHP13838

Figure 3.

Figure 3A and 3B are two line plus ribbon graphs titled gblock and Human placentas, plotting digital polymerase chain reaction percentage methylation, ranging from 20 to 100 in increments of 20 (y-axis) across Actual percentage of methylation, ranging from 20 to 80 in increments of 20 and from 40 to 70 in increments of 10 (x-axis) for Pearson correlation coefficient, respectively.

The top CpG ranked by absolute value of coefficient for all placental smoking index models was cg27402634. Measurement of cg27402634 methylation by digital PCR shows linear fit over (A) a wide range of synthetic DNA standards (r=0.995) and (B) high correlation with measurements in obtained from EPIC arrays (r=0.989) for the same samples from VCSIP. p-Values are from Pearson correlation. Numeric data can be found in Excel Tables S6 and S7. Note: CpG, cytosine–guanine dinucleotide; dPCR, digital polymerase chain reaction; EPIC, Infinium MethylationEPIC array; VCSIP, Vitamin C to Decrease the Effects of Smoking in Pregnancy on Infant Lung Function.