Table 2.
Five CpGs were selected in all three final LASSO models (EPIC, 450K, and PACE) to predict maternal smoking trained on placental DNAm data from 72 smokers and 24 never-smokers in the VCSIP randomized controlled trial.
| IlmnID | CHR | MAPINFO | Nearest gene | Feature—CpG island | Gene description |
|---|---|---|---|---|---|
| cg04233054 | 1 | 23112654 | EPHB2 | Body-shore | Ephrin type-B receptor 2 |
| cg27402634 | 3 | 156536860 | LEKR1 | IGR-shore | Leucine, glutamate, and lysine rich 1 |
| cg08103568 | 4 | 100737138 | DAPP1 | TSS1500-opensea | Dual adapter of phosphotyrosine and 3-phosphoinositides 1 |
| cg08621277 | 11 | 68271518 | SAPS3 | 5′UTR-opensea | Protein phosphatase 6 regulatory subunit 3 |
| cg07168214 | 17 | 7380112 | ZBTB4 | 5′UTR-shelf | Zinc finger and BTB domain containing 4 |
Note: The full list of selected CpGs is in Table S2. 450K, Illumina Methylation450K array; BTB, Broad-complex, Tramtrack, and Bric à brack; CHR, chromosome; CpG, cytosine–guanine dinucleotide; DNAm, DNA methylation; EPIC, Infinium MethylationEPIC array; IlmnID, unique identifier from the Illumina CG database; LASSO, least absolute shrinkage and selection operator; MAPINFO, Chromosomal coordinates of the CpG; PACE, Pregnancy And Childhood Epigenetics; VCSIP, Vitamin C to Decrease the Effects of Smoking in Pregnancy on Infant Lung Function.