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. 2024 Mar 27;36(7):2607–2628. doi: 10.1093/plcell/koae100

Figure 3.

Figure 3.

TaSAMT1 affects SA and MeSA accumulation under freezing stress. A, B) The content of endogenous SA (A) and MeSA (B) levels in wild-type Fielder, TaSAMT1-OE lines (ox-2 and ox-3), and Tasamt1-B lines (KO#1 and KO#2) under control and freezing treatment. C) Ten-day-old wild-type and Tasamt1-B (KO#1, KO#2, and KO#3) lines were sprayed with SA (500 μM) or MeSA (500 μM, 1 mM) before being exposed to −10°C for 1.5 h, followed by recovery for 24 h. Representative photographs are shown. Scale bars, 5 cm. D) Survival rates of the wild-type and Tasamt1-B lines after freezing treatment alone or together with spraying of 500 μM SA, 500 μM MeSA, or 1 mM MeSA. Data are shown as means ± SD derived from measurements of at least 15 plants per genotype in three independent experiments. E) Electrolyte leakage of the wild-type and Tasamt1-B lines in control and freezing conditions alone or together with spraying of 500 μM SA, 500 μM MeSA, or 1 mM MeSA. Data are shown as means ± SD (n = 6). The data in (D) and (E) were analyzed by one-way ANOVA followed by Tukey's test. Different lowercase letters indicate a significant difference (P < 0.05).