Table 1:
Spectroscopic parameters for proteins in this study1
| Protein | Variant | SARdenat | 15Z λdenat | 15E λdenat | 15Z λnative | 15E λnative |
|---|---|---|---|---|---|---|
| AnPixJg22 | wild-type | 0.4 | 674 | 580 | 648 | 540 |
| Cri9333_0815 | wild-type | 0.2 | 674 | 580 | 626 | 672 |
| HGZ86378 | wild-type | 0.2 | 672 | 574 | 642 | 522 |
| M595_1144 | wild-type | 0.3 | 674 | 578 | 532 | 668 |
| MBC8123355g2 | wild-type | 0.3 | 676 | 576 | 638, 586 | 450 |
| MBF2026838 | wild-type | 0.6 | 674 | 580 | 652 | 446 |
| Mic7113_1774g1 | wild-type | 0.1 | 674 | 578 | 620 | 678 |
| MP9P1_10_2A | wild-type | 0.6 | 674 | 578 | 424 | 568 |
| MP9P1_10_2B | wild-type | 0.3 | 674 | 578 | 644 | 426 |
| NIES2119_03185 | wild-type | 0.5 | 672 | 578 | 544 | 424, 616 |
| NJL97683g1 | wild-type | 0.4 | 678 | 580 | 440 | 576 |
| NJL97683g2 | wild-type | 0.1 | 674 | 584 | 408 | 564 |
| NpR4776 GAF-PHY3 | wild-type | n/r | n/r | n/r | 644 | 712 |
| NpR4776g1 | wild-type | 0.05 | 678 | 580 | 644 | 694 |
| NpR4776g1-PADCIP | D86C | 0.3 | 676 | 586 | 646 | 534 |
| OSC10802_3032 | wild-type | 0.4 | 674 | 578 | 650 | 442 |
| RfpAg13 | wild-type | n/r | n/r | n/r | 645 | 695 |
| RfpAg1-PAECIP | G79E D80C | 0.6 | 672 | 578 | 646 | 536 |
| WP_006508324 | wild-type | 0.1 | 678 | 582 | 656, 692 | 428, 434 |
| WP_027842869 | wild-type | 0.1 | 674 | 576 | 418 | 568 |
| WP_083263321 | wild-type | 0.4 | 674 | 588 | 554 | 424 |
| WP_218079506g3 | wild-type | 0.7 | 676 | 578 | 428 | 580 |
1
Reported parameters are SARdenat (specific absorbance ratio under acid denatured conditions), λdenat for both 15Z and 15E photostates (peak wavelengths from the photochemical difference spectrum of the denatured sample), and λnative (peak wavelengths for the native sample at pH 7.5, again from the photochemical difference spectrum). SAR and difference spectra were calculated as described in the Methods. All wavelengths are in nm. n/r not reported.
2
Preparation reported in [87].
3
Wavelengths and preparation reported in [21].