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. Author manuscript; available in PMC: 2024 Jul 2.
Published in final edited form as: Methods Mol Biol. 2020;2143:169–177. doi: 10.1007/978-1-0716-0585-1_13

Table 1. Table lists the chemical constituents of ACSF (1 L).

From: Ex Vivo Studies of Optic Nerve Axon Electrophysiology

ACSF (1 L) Weight Concentration
NaCl 7.3625 g 126 mM
KCl 0.2238 g 3 mM
NaH2PO4·H2O 0.1725 g 1.25 mM
MgSO4·7H2O 0.493 g 2 mM
NaHCO3 2.184 g 26 mM
Glucosea 1.8 g 10 mM
CaCl2·2H2O 2 mM
a

For ACSF0, 10 mM glucose is replaced with 10 mM sucrose