FIGURE 7.

Sulfated heparan compounds inhibit Streptococcus pneumoniae corneal infection in immunocompromised hosts and when administered after infection. Mice were made neutropenic by injecting 100 μg of anti-Ly6G antibodies at 24 and 0.5 h before injury and infection. Injured corneas were infected with 3 × 10⁸ cfu of TIGR4 and topically administered: (A) 500 ng of HS at 2, 4, or 7 h pi or (B) 500 ng of HS, NS2OS-H, or 2ODS-HP at 2 h pi and the corneal bacterial burden was assessed at 12 h pi (mean ± SE, n = 6–8, *p < 0.05). 2ODS-HP, 2-O-desulfated heparin; HP, heparin; HS, heparan sulfate; NS2OS-H, N- and 2-O-sulfated H; pi, postinfection.