Figure 10: OptoProfilin clustering response in HEK 293T cells.
A. OptoProfilin exhibits a clustering response in ATP-depleted 293T cells but not in non-ATP depleted 293T cells. In contrast to HeLa cells, a focal adhesion recruitment phenotype is absent in non-ATP depleted cells. B. Quantification of experiment described in Panel A. C. Western blot of endogenous VASP levels in HeLa and HEK 293T cells. Scale bars=10 microns.
