Figure 13: VASP phosphorylation levels in response to various cell treatments and VASP expression across cell lines.

A. Western blot of phosphorylation of VASP at Serine 157 in HeLa cells after 15 minutes of the indicated stress treatments (1. 10 mM Azide/6 mM 2-deoxyglucose; 2. 0.5 mM Sodium arsenite; 3. 200 mM Sorbitol; 4. 200 μM H₂O₂; 5. No treatment; and 6. 20 μM forskolin (used to activated adenylate cyclase and thus PKA and PKG activity)). Bar graph shows optical densitometry of bands normalized to GAPDH (Significance determined by Bonferroni t-test; ns=not significant; ***p < 0.05). B. Phosphorylation of VASP at Serine 239 in HeLa cells after 15 minutes of the indicated stress treatments (same as in panel A). Bar graph shows optical densitometry of bands normalized to GAPDH (Significance determined by Bonferroni t-test; ns=not significant; ***p< 0.05). C. Western blot of total VASP expression in HeLa, HEK 293, NIH 3T3, and Neuro-2a cell lines. Bar graph shows optical densitometry of bands normalized to GAPDH (Significance determined by Bonferroni t-test; ns=not significant; *p=0.014; ***p< 0.05).