Skip to main content
. Author manuscript; available in PMC: 2024 Jul 2.
Published in final edited form as: Chembiochem. 2024 Mar 28;25(9):e202400007. doi: 10.1002/cbic.202400007

Figure 2: Condition-dependent recruitment of OptoProfilin in HeLa cells.

Figure 2:

A. Cry2(1–531) exhibits minimal light activated response under buffer-only (top row) or ATP-depletion (second row; 10 mM NaN3/6 mM 2-DG) conditions. Upon light exposure, OptoProfilin recruits to focal adhesions under buffer-only conditions (third row) and clusters under ATP-depletion conditions (bottom row). B. Confirmation of focal adhesion recruitment by immunofluorescence of light-exposed and fixed OptoProfilin-expresssion HeLa cells (mCherry, red; Alexa 488/α-Paxillin, green). Scale bars=10 microns. C. Overlay of fluorescence intensity of mCherry (OptoProfilin) and Alexa 488 (Paxillin) at region indicated by yellow line in overlay image. Pearson’s Correlation Coefficient (calculated from six non-overlapping ROIs)=0.69 (+/−0.03).