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. Author manuscript; available in PMC: 2024 Jul 2.
Published in final edited form as: Chembiochem. 2024 Mar 28;25(9):e202400007. doi: 10.1002/cbic.202400007

Figure 3: Profilin VASP-binding mutants (S138) display different light-activated recruitment under non-stress conditions.

Figure 3:

A. OptoProfilin S138A mutant exhibits light-activated recruitment to focal adhesions in HeLa cells, but OptoProfilin S138E mutant does not. B. Quantification of percentage of cells exhibiting recruitment of mutants vs. WT OptoProfilin. C. Immunofluorescence of light-activated and fixed OptoProfilin mutants (mCherry, red; Alexa 488/VASP, green) imaged by confocal microscopy. Pearson’s Coefficients calculated from four non-overlapping ROIs (S138A=0.60 (+/−0.12) and S138E= −0.01 (+/−0.07). Scale bars=10 microns.