Skip to main content
. Author manuscript; available in PMC: 2024 Jul 2.
Published in final edited form as: Chembiochem. 2024 Mar 28;25(9):e202400007. doi: 10.1002/cbic.202400007

Figure 4: Profilin VASP-binding mutants (S138) display different light-activated recruitment under ATP-depletion conditions.

Figure 4:

A. OptoProfilin S138A mutant exhibits light-activated recruitment to clusters in ATP-depleted HeLa cells, but OptoProfilin S138E mutant does not. B. Quantification of percentage of cells exhibiting cluster recruitment of mutants vs. WT OptoProfilin. C. Immunofluorescence of light-activated and fixed OptoProfilin S138A mutant (mCherry, red; Alexa 488/VASP, green) imaged by confocal microscopy. Pearson’s Coefficients calculated from four non-overlapping ROIs=0.76 (+/− 0.07). Scale bars=10 microns.