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A. In the absence of applied stress in HeLa cells, OptoProfilin recruits to focal adhesions also labelled by VASP–mTurq. B. In the presence of applied stress (10 mM NaN3/6 mM 2-DG in DPBS), OptoProfilin rapidly sequesters VASP–mTurq into clusters. Scale bars=10 microns.
