Figure 7: Stress activated, VASP-mediated focal adhesion to cluster transition.
A. OptoProfilin recruitment to elongated focal adhesion followed by addition of ATP-depletion media (final concentration 10 mM NaN3/6 mM 2-DG) and imaging of subsequent transition to VASP-rich clusters in HeLa cells. B. Close up of transition to stress-induced phenotype. C. Quantification of the stress-induced focal adhesion transition observed in panel B. D. OptoProfilin recruitment in the absence of stress using same time course shown in panel A. Scale bars=10 microns.
