Figure 8: Light and stress activated OptoProfilin cluster contents are not consistent with traditional stress granules.
Top: SytoRNA Select stain (green) of light activated ATP-depleted OptoProfilin expressing HeLa cells (red). Pearson’s Correlation Coefficient=0.02 (+/−0.08) determined from four non-overlapping ROIs. Bottom: Ataxin2 immunofluorescence (green) of light activated ATP-depleted OptoProfilin expressing HeLa cells (red). Pearson’s Correlation Coefficient=0.01 (+/−0.06) determined from four non-overlapping ROIs. Scale bars=10 microns.
