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. 2024 Jun 20;20(6):e1011569. doi: 10.1371/journal.ppat.1011569

Fig 2. NTD antibodies with enhanced FcγR binding show increased antibody-dependent cellular cytotoxicity (ADCC).

Fig 2

(A) Antibody engineering schematic depicting wildtype (allotype G1m17) versus Fc-function enhanced antibodies (DLE, sometimes called DLE3, substitutions: S239D, A330L, I332E). (B-E) DH1052 G1m17 and DLE or (F-I) DH1050.1 G1m17 and DLE binding to immobilized mouse FcγRI, II, III, and IV measured by SPR. Antibodies were titrated to determine binding kinetics. Kinetic measurements are reported underneath the respective graph as an average of two independent experiments. Fast off-rates precluded the calculation of binding kinetics for FcγR II and III. (J) Natural killer (NK) cell-mediated antibody-dependent cellular cytotoxicity of 293T cells expressing SARS-CoV-2 WA-1. Titers are shown as % NK cells expressing the degranulation marker CD107a. Each antibody was tested at 2, 8, and 32 μg/mL and is shown ordered in the graph from lowest to highest concentration.