Table 1.

Identified fibroblast clusters from recent single-cell studies

Disease Model	Methods of Fibroblast Identification	Sequencing Information	Number of Fibroblast Clusters/Activated Clusters	Primary Conclusions
Hypertension (ANG II, mouse) (18)	Differential gene expression (PDGFRa, Col1)	10× chromium v2 kit, 12,000 cells (7,474 read from 2 hearts), Illumina Hiseq, 100,000 read/cell	9/Fibro-Wif1, Fibro-Cilp, Fibro-Thbs4	Two unique clusters: Fibro-Cilp and Fibro-Thbs4, identified after ANG II; Meox1: transcriptional switch for activation
Hypertension (ANG II/PE, mouse) (88)	FACS-sorted PDGFRa+ cells as FAP+ and FAP− cells	10× chromium v3.1 kit, Illumina NovasEq. 6,000, 100,000 reads/cell	10/Postn/Fap cluster, Cxcl1 cluster, Acta2/Tagln cluster	Activated fibroblast cluster identified with higher Postn/Fap expression; IL-1β monoclonal antibody treatment ↓ FAP+ fibroblasts
Hypertension (SHR) (17)	Col1a1+CD45−CD68−CD31−	10× chromium v3 kit, (≈2,000 cells/sample read), 1 LV per sample, n = 7 per group, Illumina Hiseq4000	9/Proinflammatory (Ccl7, Ccl2), moderately fibrogenic (Ccn3), homeostatic, gateway	Transient ACE inhibition: ↑ proportion of cells in homeostatic cluster and ↓ proportion in gateway cluster
Transverse aortic constriction (mouse) (89)	Differential gene expression	10× chromium, 35,551 total cells read (13,937 fibroblasts)	9/POSTN/FAP cluster	Fibroblasts shifted toward sham-like phenotype after JQ1 treatment
Heart failure (human) (88)	Differential gene expression/cellular indexing of transcriptomes and epitopes (CITE) proteomics	10× chromium v3.1 kit/ TotalseqA, 143,804 cells, Illumina NovasEq. 6,000 S4,104 read/cell	13/POSTN/FAP cluster, CCL2/THBS1 cluster, ACTA2/TAGLN cluster	Postn/FAP cluster: major fibrogenic cluster in heart failure samples; CCL2 cluster: proinflammatory; Runx1 was major transcription factor for fibrosis

ACE, angiotensin-converting enzyme; LV, left ventricle; SHR, spontaneously hypertensive rat.