FIG. 2.

Integrin-blocked WT cells exert more traction force than untreated WT cells on anti-CD44 antibody-coated surfaces. (a) Traction force for untreated, integrin-blocked, and CD44-blocked U251 cells on IM7-coated PAGs, which supports CD44-mediated adhesion. (b) Cell area was relatively unaffected by integrin blocking and reduced in CD44-blocked conditions on IM7-coated PAG. For untreated, CD44 blocked, integrin blocked on collagen, n = 49, 54, and 30, respectively. (c) Transmitted images (top) and traction maps (bottom) of each condition. The red line shows median. P value was calculated by the Mann–Whitney test. ^** denotes P < 0.01.