TABLE 1.
Cytokine response of EBNA1- and EBNA3C-specific T lymphocytes
| Donor | HLA class II allelea
|
Cytokine synthesisb (ng/ml)
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DRB1 | DRB3 | DRB4 | DRB5 | DQA | DQB | EBNA3C
|
EBNA1
|
|||||
| GM-CSF | IFN-γ | IL-5 | GM-CSF | IFN-γ | IL-5 | |||||||
| MK | 0404 | — | 0101 | — | 0300 | 0302 | ||||||
| 0801 | — | — | — | 0401 | 0402 | 6.383 | 19.701 | 0.096 | 5.504 | 11.968 | 3.414 | |
| TB | 1104 | 0202 | — | — | 0501 | 0301 | ||||||
| 1501 | — | — | 0101 | 0102 | 0602 | 8.545 | 11.48 | 0.39 | 5.988 | 12.911 | 5.158 | |
| CH | 0301 | 0101 | — | — | 0501 | ? | ||||||
| 1600 | — | — | 0200 | 0102 | ? | ND | 9.331 | 0.161 | ND | 10.692 | 4.384 | |
| BC1 | 0301 | 0101 | — | — | 0501 | 0201 | ||||||
| 1501 | — | — | 0101 | 0102 | 0602 | ND | 4.652 | <0.010 | ND | 8.092 | 1.111 | |
| BC2 | 0701 | — | 0101 | — | 0201 | 0201 | ||||||
| 1300 | 0301 | — | — | 0102 | 0603 | NR | NR | NR | NR | NR | NR | |
| BC3 | 0401 | — | 0101 | — | 0300 | 0302 | ||||||
| 1401 | 0202 | — | — | 0101 | 0503 | 4.21 | 3.262 | 0.361 | 3.597 | 5.259 | 2.004 | |
| BC4 | 0404 | — | 0101 | — | 0300 | 0302 | ||||||
| 1104 | — | — | — | 0501 | 0301 | NR | NR | NR | NR | NR | NR | |
| BC5 | 0400 | — | 0101 | — | 0300 | 0301 | ||||||
| 0400 | — | 0101 | — | 0300 | 0302 | 1.934 | 5.331 | 0.071 | 1.941 | 2.268 | 1.312 | |
| BC6 | 1501 | — | — | — | 0102 | ? | ||||||
| 1501 | — | — | — | 0102 | ? | 4.263 | 7.204 | 0.053 | 3.821 | 1.04 | 11.088 | |
| BC7 | ND | ND | ND | ND | ND | ND | 1.199 | 0.361 | 0.155 | 5.177 | 15.268 | 4.399 |
| BC9 | ND | ND | ND | ND | ND | ND | 0.419 | 1.391 | <0.010 | 0.783 | 1.337 | 0.384 |
| BC10 | ND | ND | ND | ND | ND | ND | 1.262 | 13.097 | 0.044 | 2.209 | 9.125 | 2.462 |
a
—, locus not expressed; ?, allele not typed.
b
Average concentration of the indicated cytokine in duplicate cultures. ND, not determined; NR, nonresponder (donor failed to demonstrate proliferative and/or cytokine responses against both EBNA1 and EBNA3C).