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. 2024 Jun 19;15:1392043. doi: 10.3389/fimmu.2024.1392043

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Copyright © 2024 Matos, Soares, Rodrigues-da-Silva, Rodolphi, Albrecht, Donassolo, Lopez-Camacho, Ano Bom, Neves, Conte, Pratt-Riccio, Daniel-Ribeiro, Totino and Lima-Junior

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Kinetics with immunization, blood collection, and euthanasia phases. (A) BALB/c mice were separated into groups and described above according to the numbering, maintained throughout the study. Each symbol is identified below with the captions. (B) BALB/c mice (n = 8 per group) were immunized intramuscularly with PvRMC-1 formulated with three adjuvants: Al(OH)3, AddaVax, and Stimune. PBS was used as a control. ELISA was performed to calculate antibody titers at all time points. Throughout the follow-up time, a continuous increase in response was observed, even in the most diluted serum samples. The group immunized with the adjuvant Stimune exhibited the most prominent response, followed by AddaVax, Antigen + PBS, and lastly Al(OH)3.