Fig. 2.

IRF1 and STAT1 mediate PD-L1 (CD274) and CXCL10 expression in tumor cells. A GEPIA based on the TCGA tumor database was used to investigate the correlation (r) between genes in patients with lung cancer adenocarcinoma, particularly the transcriptional factors IRFs and STATs with CD274, ICAM1, and CXCL10. B cBioPortal was used to further validate the correlation among IRF1, STAT1, CD274, and CXCL10 in patients with lung cancer adenocarcinoma (LUAD). (C) IRF1 and STAT1 were knocked down by shRNA and detected in 20 ng/mL of IFNα and IFNγ mixture treated A549 cells using qPCR. (D) PD-L1, CXCL10, and ISG15 were measured in 20 ng/mL of IFNα/γ mixture treated A549shLuc, A549shIRF1, and A549shSTAT1 tumor cells for 2 h using qPCR. (E) ChIP was used to investigate whether IRF1 and STAT1 were bound with the promoters of PD-L1 and CXCL10 in A549 cells. *p < 0.05