Fig. 5.

CT26 exhibits higher Ifna and Cxcl10 levels than LL/2 to potentially activate CD8⁺ T cells. A Flow cytometry was used to detect the PD-L1 expression in the selected tumor cell lines, including human lung cancer A549, mouse HCC Hepa1-6, mouse lung cancer LL/2, and mouse colon cancer CT26. B qPCR was used to compare the gene expression between LL/2 and CT26 cells. C PCR was used to validate the Ifna and Cxcl10 levels in LL/2 and CT26 cells. In addition, secreted IFNα in the LL/2 and CT26 medium (m) was captured by anti-IFNα antibodies with Dynabeads-Protein A and consequently detected using Western blots. D Activation markers Ifng, Il-2, and Cxcl10 were detected by qPCR in the mouse splenocytes treated with the culture medium (m) from LL/2 and CT-26. *p < 0.05