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. 2024 Jul 2;14:15145. doi: 10.1038/s41598-024-62897-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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5BSL2 as an example of incongruent evolution. The consensus RNA secondary structure differs from the structures into which individual sequences would fold, with sequence and structure shifted relative to each other. A helix of five nucleotides (green) in the consensus is shifted by one position in MW689971 and three positions in AY232740 and three positions in NC_009827. The nucleotides that form the middle (red) stem are shifted by four nucleotides compared to the consensus.