Table 1.

IC₅₀ values (nM ± SEM) for HDACs inhibition in HT-29 cells obtained using class-distinguishing fluorogenic substrates whole-cell assay.

	Whole-cell assay (HT-29 cells) IC50 (nM)		Isoform assay IC50 (nM)
	Class IIa	Class I/IIb	HDAC4	HDAC5	HDAC1	HDAC6	HDAC11
NT16023	46 ± 15	> 5000	0.08 ± 0.02	1.2 ± 0.17	2383	> 105	581
NT376	32 ± 7.6	> 4000	1.26	2.85	1483	5035	4851

The IC₅₀ measurements were performed in triplicates and experiments were repeated twice.