FIG. 5.

ΔICP35 mutant capsids contain wild-type levels of VP21 and VP24. (A) Dilutions of HSV-1 KOS B capsids and ΔICP35 mutant B capsids grown on Vero cells or 35J cells (expressing pre-VP22a), as indicated, were analyzed by Western blotting with antibodies against VP5, VP21/22a, and VP24. (B) Aliquots of wild-type B capsids (labeled KOS B) and C capsids (labeled KOS C) and ΔICP35 mutant capsids (ΔICP35 B) were separated on an SDS–4 to 20% PAGE gel and stained with Coomassie brilliant blue. Molecular size standards (lane MW) were electrophoresed in parallel, and the positions of the individual standards are shown. Western blots and the Coomassie-stained gel were photographed and prepared for presentation using a Kodak DCS400 digital camera and Adobe PhotoShop 5.0.