FIG. 8.

Proteins necessary for the cleavage and packaging of viral DNA are present on ΔICP35 capsids. (A) Twofold dilutions of wild-type B capsids and ΔICP35 B capsids were analyzed by Western blotting with antibodies to VP5 and VP23 (upper panel), the UL6 protein (center panel), and the UL25 protein (lower panel). (B) Wild-type B and C capsids and ΔICP35 mutant capsids were analyzed by Western blotting with an antiserum raised against the UL15 protein. The left panel shows the reactivity of B and C capsids with the UL15 serum. The right panel shows capsids from ΔICP35 virus grown on Vero cells and complementing 35J Vero cells that express UL26.5. Western blots were photographed using a Kodak DCS400 digital camera and Adobe PhotoShop 5.0.