TABLE 2.
UL25 protein levels in ΔICP35 mutant capsids and infected cellsa
Protein | Relative protein level (% of control)
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Expt 1
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Expt 2
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Expt 3
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Capsids | Cells | Capsids | Cells | Capsids | Cells | |
VP5 | 100 | NDb | 100 | 100 | 100 | 100 |
VP23 | 125 | ND | 77 | 85 | 73 | 66 |
UL25 | 279 | ND | 403 | 134 | 287 | 76 |
Protein levels were determined as described in Materials and Methods and are presented as the percentage of each protein in ΔICP35 capsids (capsids) or total infected cell lysates (cells) relative to that found in HSV-1 KOS B capsids or HSV-1 KOS total infected-cell lysates, examined in parallel. Comparisons between samples and experiments were performed by setting levels for HSV-1 KOS to 100%. Comparisons within samples were performed by setting levels of VP5 to 100%.
ND, not done.