Fig. 1. Multiplexed single-cell sequencing of stressed primary human islets.

a Schematic for multiplexing single-cell RNA sequencing of primary human islets with exogenous stressors. b UMAP showing which cells were treated with each stressor, and a bar graph of the ratio of each treatment in the entire population. c UMAP of islet cell populations based on RNA expression, and a bar graph of the ratio of cell types in the entire population. d Heatmap of key markers used to identify cell types in UMAP. e UMAP of key endoplasmic reticulum and inflammatory stress markers. Source data are provided as a Source Data file.