Fig. 5. Six genes are upregulated across endocrine cell types and stressors.

a Upregulated DEG in β-, α-, and δ-cells across BFA, TG, and CM from 4a. Genes that were in at least two cell types were used to make a new Venn diagram across stressors. b Heatmap of the expression of the 6 upregulated shared genes in CTRL, BFA, TG, and CM. c >3 of the shared 6 genes were found downstream of these regulons in β-, α-, and δ-cells. d Heatmap of genes that activate/inhibit CIB1 in β-cells. e SC-islet differentiation with the addition of lentiviral short hairpin RNA (shRNA) or open reading frame (ORF) targeting CIB1. f Static glucose-stimulated insulin secretion of CTRL and CIB1 KD (n = 4). The left two bars are CTRL, and the right two bars are CIB1 KD. Paired t test for 2 mM vs. 20 mM (CTRL P = 0.0067, KD P = 0.0249), Unpaired t test for 2 mM CTRL vs. 2 mM KD (P = 0.001) or 20 mM CTRL vs. 20 mM KD (P = 0.0057). g Caspase 3/7 assay of CIB1 KD and CIB1 OE under stress conditions, BFA (n = 8; KD P = 0.00048; OE P = 0.000211), CM (n = 5; KD P = 1.1 × 10⁻⁶), and TG (n = 8; KD P = 6.87 × 10⁻⁶), unpaired t test, **p < 0.01,***p < 0.001,****p < 0.0001, and not significant (ns) by one-sided paired t test. All error bars represent s.e.m. Source data are provided as a Source Data file.