FIG. 1.

cDNA cloning strategy for mild CTV isolate sequences. (A) Diagram of the organization of the CTV T30 genome (19,259 nt). PRO, papain protease-like domain; MT, methyltransferase-like domain; HEL, helicase-like domain. (B, top panel) Schematic representation of the localization of the oligo(dT)-primed random T30 cDNA clones, the naturally occurring dRNA in isolate T30 (DI) (M. Mawassi et al., unpublished data), and the cDNA clone T308 (20) used to design T30-specific primers. (B, bottom panel) Schematic representation of the localization and size of the eight overlapping RT-PCR T30 cDNA clones used to determine the T30 genomic sequence. (C) Schematic representation of the localization of RT-PCR CTV clones A and B and locations (solid boxes) of the four hypervariable regions sequenced for comparing CTV isolates B252, B272, and B354 with T30 and T385. These CTV cDNA clones were obtained as indicated in Material and Methods.