TABLE 2.

RT-PCR primers used to generate specific CTV cDNA clones

Forward primer	Location (nt)	Cloning site	Reverse primer	Location (nt)	Cloning site	cDNA clone generated
C198	1–19	HindIII	C227	2427–2449	NcoI	6
C226	2402–2430	NdeI	C258	5169–5193	Blunt	5b
C257	5077–5097	NdeI	C225	8654–8677	NcoI	5a
C229	8631–8654	HindIII	C228	9460–9492	BamHI	4
C213	9439–9457	BamHI	C239	12625–12648	XhoI	3b
C240	12562–12584	BamHI	C214	14766–14787	XhoI	3a
C215	14746–14766	HindIII	C216	18079–18100	XhoI	2
C217	17903–17922	XhoI	C118	19237–19259	NcoI	1
C326	1–28	Blunt	C323	3686–3706	NotI	A
C295	8291–8310	Blunt	C225	8630–8654	Blunt	B