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Experimental design of the study. DMSO: dimethyl sulfoxide (ferrostatin dissolved in dimethyl sulfoxide but morphine dissolved in saline), i.p.: intraperitoneally, s.c.: subcutaneousl, GPX4: glutathione peroxidase 4, Nrf2: nuclear factor erythroid 2-related factor 2, TAS: total antioxidant status, TOS: total oxidant status.
