FIG. 4.

Deglycosylation of I-E2 does not influence its binding activity. I-E2₇₁₅ was digested with α-mannosidase or with endo H. MOLT-4 binding activity of α-mannosidase-treated (solid circles) and endo H-treated (solid squares) E2 was compared to that of mock-treated E2 (open symbols). Cell-bound E2 was measured by flow cytometry using chimpanzee anti-E1-E2 antiserum and is indicated as net MFI.