FIG. 5.

Diagram of the E2 constructs used for DNA immunization. The relevant HCV proteins are shown at the top. The black box corresponds to the signal peptide at the C terminus of E1 directing E2 into the ER, and terminal amino acid positions in the constructs are indicated. HCV sequences present in each construct are indicated by black lines which are drawn to scale and oriented with respect to the HCV proteins shown at the top. Grey boxes represent an N-terminal 25-aa TPA-derived signal peptide directing E2 into the ER, the white box indicates three additional C-terminal amino acids leading to the motif KDEL, and the hatched box indicates the C-terminal cytoplasmic tail of the adenovirus E3/19K protein (SRRSFIDEKKMP).