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E2 stably expressed from the different DNA immunization constructs in CHO cells is endo H sensitive. E2 was immunoprecipitated from cell lysates with chimpanzee anti-E1-E2 antiserum and treated with endo H (+) or left untreated (−). The samples were analyzed by SDS-PAGE (10% acrylamide) under reducing conditions and Western blotting. Arrows indicate the presence of two closely migrating bands. Sizes (in kilodaltons) of the molecular mass markers are indicated on the left.
