Table 1.

Overview of urine specimen and rapid test performance to detect CTX-M

	NR	Sample type	Leukocyte count (per µL)	Polymicrobial	Enterobacteralesa			Other species	NG-Test® CTX-M MULTI			ESBL confirmation (genotypic and phenotypic)
					species	quantification	ESBL phenotypeb		centrifugation	filtration	direct from colony	growth on ESBL plate	WGS/CTX-M	MLST
suspected ESBL	UR1	urine	<10	yes	E. coli	10^6 cfu/ml	yes	Enterococcus faecalis, Acinetobacter species	pos	pos	pos	yes	CTX-M 15	ST131
	UR2	catheter urine	10–25	no	E. coli	10^4 cfu/ml	yes	—	pos	pos	n/a	n/a	n/a	n/a
	UR3	urine (bladder puncture)	<10	no	E. coli	10^4 cfu/ml	yes	—	pos	pos	pos	yes	CTX-M 27	ST131
	UR4	midstream urine	10–25	yes	E. coli	10^4 cfu/ml	yes	E. faecalis	pos	pos	pos	yes	CTX-M 15	ST131
	UR5	catheter urine	>500	yes	E. coli	10^6 cfu/ml	yes	E. faecalis, Streptococcus anginosus	pos	pos	pos	yes	CTX-M 15	ST131
	UR6	catheter urine	>500	no	Citrobacter braakii	10^4 cfu/ml	yes	—	neg	neg	neg	yes	no CTX-M detected, CMY 101	ST561
	UR7	midstream urine	>500	no	E. coli	10^6 cfu/ml	yes	—	pos	pos	pos	yes	CTX-M 1	ST88*
	UR8	midstream urine	10–25	no	E. coli	10^5 cfu/ml	yes	—	negc	neg	pos	yes	CTX-M 15	ST131
	UR9	catheter urine	75	yes	E. coli	10^5 cfu/ml	yes	Aerococcus urinae	pos	pos	pos	yes	CTX-M 15	ST349
	UR10	midstream urine	>500	yes	E. coli	10^6 cfu/ml	yes	urogenital flora	pos	pos	pos	yes	CTX-M 1	ST88
	UR11	catheter urine	10–25	yes	E. coli	10^6 cfu/ml	yes	urogenital flora	pos	pos	pos	yes	CTX-M 15	ST131
	UR12	catheter urine	75	yes	E. coli	10^6 cfu/ml	yes	Klebsiella pneumoniae, Candida krusei	neg	neg	neg	yes	no CTX-M detected	ST131-1LV
	UR13	midstream urine	>500	no	E. coli	10^6 cfu/ml	yes		pos	pos	pos	yes	CTX-M 1	ST2015
	UR14	midstream urine	75	yes	E. coli	10^6 cfu/ml	yes	urogenital flora	neg	neg	neg	yes	no CTX-M detected	ST167
	UR15	catheter urine	>500	yes	K. pneumoniae	10^6 cfu/ml	yes	Morganella morganii	negc	neg	weak pos	yes	CTX-M 15	ST432
	UR16	midstream urine	>500	yes	E. coli	10^6 cfu/ml	yes	urogenital flora	pos	pos	pos	yes	CTX-M 65	ST69
	UR17	midstream urine	<10	yes	E. coli	10^6 cfu/ml	yes	Aerococcus urinae	pos	pos	pos	yes	CTX-M 15	ST4891
	UR18	midstream urine	>500	yes	K. pneumoniae	10^5 cfu/ml	yes	Staphylococcus epidermidis	pos	pos	pos	yes	CTX-M 15	ST1662
	UR19	catheter urine	<10	no	E. coli	10^6 cfu/ml	yes		pos	pos	pos	yes	CTX-M 15	ST361
	UR20	midstream urine	75	no	E. coli	10^6 cfu/ml	yes		pos	pos	pos	yes	CTX-M 15	ST1193
non-ESBL	UR21	midstream urine	75	no	E. coli	10^4 cfu/ml	no	—	neg	neg	n/a	no	n/a	n/a
	UR22	midstream urine	10–25	no	E. coli	10^6 cfu/ml	no	—	neg	neg	n/a	no	n/a	n/a
	UR23	midstream urine	75	no	E. coli	10^6 cfu/ml	no	—	neg	neg	n/a	no	n/a	n/a
	UR24	midstream urine	10–25	no	E. coli	10^6 cfu/ml	no	—	neg	neg	n/a	no	n/a	n/a
	UR25	midstream urine	75	no	E. coli	10^6 cfu/ml	no	—	neg	neg	n/a	no	n/a	n/a
	UR26	midstream urine	75	no	E. coli	10^6 cfu/ml	no	—	neg	neg	n/a	no	n/a	n/a
	UR27	midstream urine	75	no	E. coli	10^6 cfu/ml	no	—	neg	neg	n/a	no	n/a	n/a
	UR28	midstream urine	>500	no	E. coli	10^6 cfu/ml	no	—	neg	neg	n/a	no	n/a	n/a
	UR29	midstream urine	>500	no	E. coli	10^5 cfu/ml	no	—	neg	neg	n/a	no	n/a	n/a
	UR30	catheter urine	>500	yes	E. coli	10^6 cfu/ml	no	Enterococcus faecalis	neg	neg	n/a	no	n/a	n/a
	UR31	catheter urine	75	no	E. coli	10^6 cfu/ml	no	—	neg	neg	n/a	no	n/a	n/a
	UR32	midstream urine	>500	no	E. coli	10^5 cfu/ml	no	—	neg	neg	n/a	no	n/a	n/a
	UR33	midstream urine	<10	yes	urogenital flora	10^2 cfu/ml	no	—	neg	neg	n/a	no	n/a	n/a
	UR34	catheter urine	>500	yes	P. mirabilis	10^5 cfu/ml	no	Enterococcus faecalis	neg	neg	n/a	no	n/a	n/a
	UR35	midstream urine	75	no	E. coli	10^5 cfu/ml	no	—	neg	neg	n/a	no	n/a	n/a
	UR36	midstream urine	<10	yes	E. coli	10^6 cfu/ml	no	Enterobacter cloacae	neg	neg	n/a	no	n/a	n/a
	UR37	catheter urine	10–25	yes	K. pneumoniae	10^6 cfu/ml	no	Staphylococcus aureus	neg	neg	n/a	no	n/a	n/a
	UR38	midstream urine	<10	no	E. coli	10^3 cfu/ml	no	urogenital flora	neg	neg	n/a	no	n/a	n/a
	UR39	catheter urine	75	no	E. coli	10^4 cfu/ml	no	none	neg	neg	n/a	no	n/a	n/a
	UR40	catheter urine	75	no	E. coli	10^6 cfu/ml	no	none	neg	neg	n/a	no	n/a	n/a

ESBL, extended-spectrum beta-lactamase; WGS, whole-genome sequencing; MLST, multi-locus sequence type; pos, positive; neg, negative; n/a, not applicable.

^aData acquired from the routine microbiological diagnostics.

^bIsolates with suspected ESBL phenotype were defined as Enterobacterales with phenotypic resistance to penicillins and second- and third-generation cephalosporins, as determined by VITEK^®2.

^cDiscrepant results between direct detection of CTX-M from urine samples and from bacterial colony are indicated in bold.