TABLE 5.
Replication of SIV and HIV-1 isolates in PBMC from wild-type and Δ32-CCR5 donors and in CEMx174 cells in two different experiments
| SIV or HIV-1 isolate | TCID50 | Virus (ng of p24 or p27 antigen/ml) produced in the following cells on the indicated day postinfectiona:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Wild-type PBMC (D)
|
Δ32-CCR5 PBMC (1)
|
Δ32-CCR5 PBMC (2)
|
Wild-type PBMC (E)
|
Δ32-CCR5 PBMC (3)
|
|||||||
| 7 | 11 | 7 | 11 | 7 | 11 | 7 | 11 | 7 | 11 | ||
| SIVmac239 | 100 | 10.9 | 87.7 | 0.2 | 0.5 | 3 | 44.2 | 34 | 383 | 8.7 | 201 |
| 500 | 27.4 | 112 | 0.5 | 0.8 | 7.7 | 53.4 | 134 | 460 | 112 | 298 | |
| 1000 | 37.6 | 105 | 1.6 | 3.4 | 7.6 | 52 | ND | ND | ND | ND | |
| SIVmac251 | 100 | 4.4 | 146 | 0.5 | 1.4 | 0.6 | 9.9 | 85 | 481 | 46 | 309 |
| SIVrcm | 100 | 154 | 707 | 65 | 710 | 55 | 637 | ND | ND | 294 | 358 |
| SF162 | 100 | 18.1 | 16.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
a
Two experiments are recorded: one that compared donors D, 1, and 2 and the other that compared donors E and 3 (donor designations in parentheses after cell types). ND, not done.