. 2024 Jun 10;21(7):3310–3320. doi: 10.1021/acs.molpharmaceut.4c00063

Table 1. Validation Parameters Including Sensitivity, Linearity, Accuracy, and Precision for the HPLC/UV Method Developed for the Detection and Quantification of Siponimod^a.

		CC₁	CC₂
concentration range		0.05–0.5 μg/mL	1–20 μg/mL
slope		267.01 ± 9.50	267.25 ± 3.63
intercept		–1.35 ± 0.81	2.74 ± 18.02
LOD (μg/mL)		0.01	0.22
LOQ (μg/mL)		0.03	0.67
accuracy (% recovery)	intraday	98.37–101.42	98.05–102.84
	interday	99.30–104.44	98.66–100.68
precision (% RSD)	intraday	1.8–6.7	1.54–3.85
	interday	1.5–7	1.13–5.68

LOD: limit of detection, LOQ: limit of quantitation, and % RSD: percentage relative standard deviation. Slope and intercept values are presented as mean ± standard deviation.

Table 1. Validation Parameters Including Sensitivity, Linearity, Accuracy, and Precision for the HPLC/UV Method Developed for the Detection and Quantification of Siponimoda.

Table 1. Validation Parameters Including Sensitivity, Linearity, Accuracy, and Precision for the HPLC/UV Method Developed for the Detection and Quantification of Siponimod^a.