Table 3. PK Parameters of Siponimod in Albino Rabbits, Estimated Using NCA^a.

parameter	low dose	high dose
dose (ng)	1300	6500
R	–0.99	–0.96
Cmax (ng/mL)	515.56 ± 42.57	3161.68 ± 387.11
Tmax (h)	1	0.5
T1/2 (h)	2.80	3.88
Vd (mL)	2.15	2.08
AUC0–∞ (ng/mL h)	2188.29 ± 49.51	15485.20 ± 1444.10
CL (mL/h)	0.59	0.42

^aC_max is the maximum siponimod concentration in the vitreous after intravitreal injection. T_max is the time of C_max, T_1/2 is the elimination half-life in hours (h), V_d is the observed volume of distribution at the steady state, AUC_0–∞ is the area under the curve, and CL is the observed clearance from the vitreous. C_max is presented as mean ± standard deviation. AUC_0–∞ is presented as mean value ± standard deviation estimated using previously reported methods for calculating standard deviation for PK studies with destructive measurement techniques.⁴¹⁻⁴³