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Kinetics of ascorbate oxidation monitored at A265 in the presence of 5 μM CuCl2 alone and in the presence of 50 μM His-Leu, histidine (His), or histamine (Hstm). The measurements were performed for 100 μM AscH– in 50 mM HEPES (pH 7.4). The inset represents the initial ascorbate oxidation rate calculated by linear fitting of the data between the 13th and 14th minutes of the measurement.
