Figure 1.
Insulin signaling in R67D01-labeled DANs is required for PIFI
(A) The diagram of pre-feeding paradigm. During pre-feeding, tryptone (T) was used as the protein food, sucrose (S) as the sugar food, and agar (A) as the no-pre-feeding control. Normal food (NF) was used in the test.
(B–D) Knocking down InR in R67D01 neurons abolished the feeding inhibition effect induced by protein pre-feeding (PIFI) but not sugar pre-feeding (SIFI). The suppression index of protein pre-feeding (SIT) in these InR-KD flies was significantly decreased (C), whereas that of sugar pre-feeding (SIS) was not impaired (D). n = 6–8.
(E and F) In hungry flies, expressing InRCA in R67D01 neurons reduced food consumption of normal food and tryptone but not sucrose. n = 6–10.
(G) The expression pattern of R67D01-Gal4. Dots symbolize the R67D01-labeled neurons, and the red dot represents the insulin-responding neurons that are required for PIFI.
(H–J) InR KD in R67D01ΘIPC neurons (with no expression in IPCs, H) abolished the PIFI effect (I). n = 6–12.
(K–-M) InR KD in R67D01ΘTH neurons (with no expression in DANs, K) did not affect the PIFI (L) or SIFI (M) effect. n = 8–12.
n represents the number of trials. Student’s t test for relative food consumption (RFC) in (B), (I), (J), (L), and (M). One-way ANOVA, Dunnett test for suppression index (SI) in (C), (D), (I), (J), (L), and (M) and for RFC in (F). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. n.s. indicates no statistical significance. The data are shown in mean ± SEM. Scale bar, 100 μm.
See also Figure S1.