Figure 2.

Insulin signaling in T1-DANs is required for the PIFI effect

(A) The PIFI effect was significantly reduced in flies with InR KD in T1-DANs using T1-Gal4. n = 12–18.

(B) InR KD in PPM3-DANs did not affect PIFI effect. n = 6.

(C) InR KD in T1-DANs by T1′-Gal4 suppressed the PIFI effect. n = 7–10.

(D and E) The PIFI effect was significantly decreased when expressing InR^DN in T1-DANs using T1- or T1′- Gal4 strains. n = 5 in (D). n = 6–8 in (E).

(F) The flow diagram of temperature-induced gene expression. Flies were collected at hatch, cultivated in NF at 18°C for 2 days, and then transferred to 29°C to induce gene expression. After another 2 days, flies were transferred to agar for 24 h starvation (12 h at 29°C and 12 h at 25°C) and were subjected to the feeding test at 25°C.

(G and H) Expressing InR^CA in T1-DANs during adult stage reduced food consumption of tryptone but not sucrose. n = 5–8.

n represents the number of trials. Student’s t test for RFC in (A)–(E). One-way ANOVA, Dunnett test for SI in (A)–(E) and for RFC in (G) and (H). ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001. n.s. indicates no statistical significance. The data are shown in mean ± SEM. Scale bar, 100 μm.

See also Figure S2.