Figure 4.
PIFI is abolished when T1-DANs are inhibited
(A) The diagram of optogenetic manipulation in the pre-feeding paradigm.
(B–E) Optogenetic silencing T1-DANs using T1-Gal4 (B and C) or T1′-Gal4 (D and E) abolished the PIFI effect but not the SIFI effect. n = 5–9. Student’s t test for RFC. One-way ANOVA, Dunnett test for SI.
(F and G) Opto-inhibiting T1-DANs abolished the feeding inhibition induced by activating IPCs selectively for protein-containing foods, normal food (n = 5–7) and tryptone (n = 6–9), but not for sucrose (n = 6–7). The same set of data was used for the first and seventh columns in all three groups of experiments. One-way ANOVA, Dunnett test for the comparison within groups. See also Table S2 for two-way ANOVA comparison between groups.
n represents the number of trials. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. n.s. indicates no statistical significance. The data are shown in mean ± SEM.