Activation of T1-DANs suppresses protein consumption
(A–C) Optogenetic activation of T1-DANs using two Gal4 stains both suppressed food consumption of tryptone but not sucrose. RFC, relative feeding consumption. n = 5–9 for tryptone and n = 11–13 for sucrose in (B). n = 7–9 for tryptone and n = 6–7 for sucrose in (C).
(D and E) In the two-choice assay, the choice ratio of protein-containing food was significantly reduced when activating T1-DANs, while it was unchanged when PPM3-DANs were activated. n = 5–11.
(F and G) Optogenetic activation of T1-DANs reduced protein choice in flies with 24 h protein deprivation. n = 10.
n represents the number of trials. One-way ANOVA, Dunnett test. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. n.s. indicates no statistical significance. The data are shown in mean ± SEM.
See also Figure S5.