PB-LNs are required for PIFI
(A and B) Optogenetic silencing of PB-LNs suppressed PIFI but did not affect SIFI. n = 5–6. Student’s t test for RFC. One-way ANOVA, Dunnett test for SI.
(C and D) Optogenetic activation of PB-LNs inhibited both tryptone and sucrose feeding. n = 6–9. One-way ANOVA, Dunnett test.
(E) The illustration of the neural circuits downstream of the insulin signal that is required for PIFI.
n represents the number of trials. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. n.s. indicates no statistical significance. The data are shown in mean ± SEM.
See also Figure S7.