REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-TH (dilution 1:200)	Millipole	Cat#AB152; RRID: AB_390204
Mouse anti-HA (dilution 1:1000)	ABclonal	Cat#AE008; RRID: AB_2770404
Goat-anti-rabbit 633 (dilution 1:1000)	Invitrogen	Cat#A-21071; RRID: AB_141419
Goat-anti-mouse (dilution 1:1000)	Invitrogen	Cat#A-21052; RRID: AB_2535719
Mouse anti-Flag (dilution 1:400)	ABclonal	Cat#AE005, RRID: AB_2770401
Rabbit anti-HA (dilution 1:10000)	Cell signaling	Cat#3724S, RRID:AB_1549585
Rabbit-HRP (dilution 1:1000).	CWbio	Cat#cw0103s, RRID:AB_2814709
Chemicals, peptides, and recombinant proteins
Agar (w/v 1%)	Solarbio	A8190
Tryptone (w/v 1.7%)	OXOID	LP0042
Sucrose (w/v 10%)	Sinopharm Chemical Reagent	10021418
Brilliant blue	Care	N/A
Sulforhodamine B	sigma	341738
all-trans-retina (ATR)	sigma	R2500
ATP (2.5 mM)	Merck	A1852
TTX (1 μM)	TaiZhou KangTe	N/A
SCH 23390 hydrochloride (100 μM)	TOCRIS	0925
Alexa Fluor 568 hydeazide	Invitrogen	A10441
PFA	EMS	157–8
PBS	Sangon Biotech	B548117
GS	Gibco	16210–072
Triton X-100	Merck	11869
DMSO (10 mM)	sigma	D8418
Experimental models: Organisms/strains
UAS-TH-miR#G	Xie et al.89	N/A
UAS-Dop1R1-miR	Xie et al.89	N/A
UAS-Dop1R2-miR	Xie et al.89	N/A
TH-C-Gal4DBD	Xie et al.89	N/A
DAT-B-Gal4DBD	Xie et al.89	N/A
R55C01-Gal4DBD	Xie et al.89	N/A
R60C07-Gal4DBD	Xie et al.89	N/A
R76F01-Gal4DBD	Xie et al.89	N/A
R76F05-Gal4DBD	Xie et al.89	N/A
TH-Gal80	Berry et al.90	N/A
dilp2-LexA	Li and Gong.91	N/A
ilp21, gd2HF	Park et al.45	N/A
w1118	Bloomington Drosophila Stock Center (BDSC)	5905
R67D01-Gal4	Bloomington Drosophila Stock Center (BDSC)	39412
VT029577-p65AD; VT038817-Gal4DBD	Bloomington Drosophila Stock Center (BDSC)	86626
R55G08-Gal4	Bloomington Drosophila Stock Center (BDSC)	50422
R52B01-Gal4	Bloomington Drosophila Stock Center (BDSC)	38820
R60D05-Gal4	Bloomington Drosophila Stock Center (BDSC)	39247
R37F06-Gal4	Bloomington Drosophila Stock Center (BDSC)	49962
R55G08-LexA	Bloomington Drosophila Stock Center (BDSC)	53544
R52B01-LexA	Bloomington Drosophila Stock Center (BDSC)	52826
R60D05-LexA	Bloomington Drosophila Stock Center (BDSC)	52867
R37F06-LexA	Bloomington Drosophila Stock Center (BDSC)	52764
LexAop-Gal80	Bloomington Drosophila Stock Center (BDSC)	32216
dilp2-Gal4	Bloomington Drosophila Stock Center (BDSC)	37516
tub-Gal80ts	Bloomington Drosophila Stock Center (BDSC)	7018
UAS-InR-CA41	Bloomington Drosophila Stock Center (BDSC)	8263
UAS-InR-DN41	Bloomington Drosophila Stock Center (BDSC)	8252
UAS-CsChrimson	Bloomington Drosophila Stock Center (BDSC)	55135
UAS-GtACR2	Bloomington Drosophila Stock Center (BDSC)	92987
LexAop-CsChrimson	Bloomington Drosophila Stock Center (BDSC)	55138
UAS-CaMPARI	Bloomington Drosophila Stock Center (BDSC)	58761
UAS-jGCaMP7s	Bloomington Drosophila Stock Center (BDSC)	79032
LexAop-P2X2	Bloomington Drosophila Stock Center (BDSC)	76030
UAS-P2X2	Bloomington Drosophila Stock Center (BDSC)	91223
LexAop-jGCaMP7s	Bloomington Drosophila Stock Center (BDSC)	80913
UAS-mGFP	Bloomington Drosophila Stock Center (BDSC)	5137
UAS-sytGFP	Bloomington Drosophila Stock Center (BDSC)	6925
UAS-DenMark	Bloomington Drosophila Stock Center (BDSC)	33061
UAS-CD4-spGFP1-10, LexAop-CD4-spGFP11	Bloomington Drosophila Stock Center (BDSC)	58755
UAS-nSyb-spGFP1-10, LexAop-CD4-spGFP11	Bloomington Drosophila Stock Center (BDSC)	64314
UAS-InR-RNAi	Vienna Drosophila RNAi Center (VDRC)	V992
UAS-D2R-Ri	Vienna Drosophila RNAi Center (VDRC)	V11471
Oligonucleotides
Generate R67D01-p65AD: forward primer 5′- cgaaaagtgccacctgacgtcAGAAGGGGCTTTTGCAAGAAC	Integrated DNA Technologies	N/A
Generate R67D01-p65AD: reverse primer 5′- tccccgggcgagctcggccggccCCCT TGGGCCGCAATTAA	Integrated DNA Technologies	N/A
Recombinant DNA
Plasmid: R67D01-p65AD	This paper	N/A
pBPp65ADZpUw vector	Addgene	26234
Software and algorithms
FIJI	ImageJ (Schneider et al.)88	https://imagej.net/ij/download.html
GraphPad Prism 8	GraphPad Software	https://www.graphpad.com
Excel	Microsoft	https://www.microsoft.com/en-us/microsoft-365/excel